Postdoctoral Fellow - Protein Engineering
- City of Hope
- Location: Duarte, CA
- Job Number: 7079076 (Ref #: 10011000)
- Posting Date: Mar 22, 2022
- Application Deadline: Open Until Filled
About City of Hope
City of Hope is an independent biomedical research and treatment organization for cancer, diabetes and other life-threatening diseases.
Founded in 1913, City of Hope is a leader in bone marrow transplantation and immunotherapy such as CAR T cell therapy. City of Hope’s translational research and personalized treatment protocols advance care throughout the world. Human synthetic insulin, monoclonal antibodies and numerous breakthrough cancer drugs are based on technology developed at the institution. AccessHope™, a subsidiary launched in 2019 serves employers and their health care partners by providing access to City of Hope’s specialized cancer expertise.
A National Cancer Institute-designated comprehensive cancer center and a founding member of the National Comprehensive Cancer Network, City of Hope is ranked among the nation’s “Best Hospitals” in cancer by U.S. News & World Report and received Magnet Recognition from the American Nurses Credentialing Center. Its main campus is located near Los Angeles, with additional locations throughout Southern California and in Arizona.
City of Hope’s commitment to Diversity, Equity and Inclusion
We believe diversity, equity and inclusion is key in serving our mission to provide compassionate patient care, drive innovative discovery, and advance vital education focused on eliminating cancer and diabetes in all of our communities. Our commitment to Diversity, Equity and Inclusion ensures we bring the full range of skills, perspectives, cultural backgrounds and experiences to our work -- and that our teams align with the people we serve in order to build trust and understanding. We are dedicated to fostering a community that embraces diversity - in ideas, backgrounds and perspectives; this is reflected in our work and represented in our people.
The Williams lab is looking for a Postdoctoral Fellow interested in seeing their efforts translated to the clinic to treat cancer and diabetic patients. The position involves an atoms-up approach to design novel biologics to mitigate on-target, off-tissue toxicities and improve their therapeutic efficacy. The requisite research includes the application of structural biology, computation, biophysics, in vitro and in vivo assays while communicating and coordinating these efforts with the rest of the team and clinicians at City of Hope focusing on immediate clinical needs. The successful candidate will be deeply involved through all aspects of this bench-to-bedside approach.
The Williams lab is equipment with state-of-the-art instruments including multiple incubators, biosafety cabinets, multiple FPLCs (including a new Biorad NGC FPLC – automating the purification of dozens of molecules per day), an Agilent HPLC, Biacore T200, Waters nanoITC, Beckman XLI, Mosquito crystallization robot, Formulatrix imagers, and a Rigaku 007HF diffractometer. A new Sony SH800 cell sorter is also located in the lab to further drive these efforts using novel display libraries developed in-house. In addition, the successful candidate will have access to 27 core facilities across the campus.
Basic education, experience and skills required for consideration:
PhD in chemistry, biochemistry, protein chemistry, biochemical engineering or related field with 1 to 3 years of postdoctoral experience
Experience with protein expression and purification
Fully versed in protein purification methods (Affinity, SEC, IEX), analysis (e.g., SDS-PAGE)
Experience with cell culture (E. coli and CHO a must, yeast and insect desirable). Experience with SPR and other biophysical methods desirable
Protein engineering, production, purification in CHO
Biophysical characterization (SPR, ITC, AUC, CD)
Assay development, interpretation, and troubleshooting
Excellent written and verbal communication skills
Good problem-solving skills
Ability to conduct independent research
Ability to supervise others and work effectively in a team
Preferred education, experience and skills:
Enzymology experience a plus
CryoEM experience a plus
City of Hope is a world leader in translational medicine, including synthesis of first synthetic gene, humanization of monoclonal antibodies and pioneering work in CAR T cells. These discoveries have led to some of the most important therapeutics in world including recombinant insulin for maintenance of type 2 diabetes and Rituxan, Herceptin, Avastin, etc. for the treatment of cancer. Building off these successes, City of Hope houses three FDA approved GMP facilities along with its 27 core facilities to rapidly advance discoveries.
For more information about Dr. Williams' research, please click here.
As a condition of employment, City of Hope requires staff to comply with all state and federal vaccination mandates.
City of Hope is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, or status as a qualified individual with disability.
City of Hope is a community of people characterized by our diversity of thought, background and approach, but tied together by our commitment to care for and cure those with cancer and other life-threatening diseases. The innovation that our diversity produces in the areas of research, treatment, philanthropy and education has made us national leaders in this fight. Our unique and diverse workforce provides us the ability to understand our patients' needs, deliver compassionate care and continue the quest for a cure for life-threatening diseases. At City of Hope, diversity and inclusion is a core value at the heart of our mission. We strive to create an inclusive workplace environment that engages all of our employees and provides them with opportunities to develop and grow, both personally and professionally. Each day brings an opportunity to strengthen our work, leverage our different perspectives and improve our patients’ experiences by learning from others. Diversity and inclusion is about much more than policies and campaigns. It is an integral part of who we are as an institution, how we operate and how we see our future.