Postdoctoral Research Assistant
- Lewis Katz School of Medicine Temple University
- Location: Philadelphia, PA
- Job Number: 7084890
- Posting Date: Sep 8, 2021
- Application Deadline: Open Until Filled
Job DescriptionThis position is sponsored by grant from the NIH’s Martin Delaney Collaboratories for HIV Cure Research Program, titled “CRISPR for Cure”. It is a minimum 1-year position extendable to up to 5 years. Starting salary about $54,000 plus benefits.
The project is a combination of gene therapy/gene-editing, virology, and immunology research fields.
HIV-specific cytotoxic T cells (CTLs) can play an important role in clearing viral reservoirs and controlling viral rebound after analytical treatment interruption (ATI). However, functional exhaustion of viral-specific CTLs cannot be fully restored by antiviral therapy (ART). In this project, we will use CRISPR to modulate PD-1 and IL-15Rα expression in HIV-specific CTLs. In addition, we will use CRISPR to increase IL-15 production in myeloid cells, such as macrophages and dendritic cells. We will use the same strategy on NK cells, because the PD-1 and IL-15 signaling pathways are share between CTLs and NK cells. The goals of the project are:
1) Design of CRISPR-dCas9 based transcriptional activation strategies and selection and testing of candidate gRNAs.
2) Ex vivo validation of dCas9-effector-mediated transcription activation of target genes in primary CD8+T and NK cells
3) Off-target analysis of the final CRISPR gRNAs.
To undertake research under the guidance of the Principal Investigator and support the supervision of any students who may be assisting with the research.
Main Duties and Responsibilities:
• To independently undertake research in the relevant discipline area using the appropriate methods and/or techniques.
• To produce research reports and/or publications as required by the funding body or for dissemination to the wider academic community.
• To collaborate with research colleagues and support staff internally as appropriate to the research and to actively develop appropriate external contacts and networks relevant to the project and to future funding opportunities.
• To provide guidance and support to any students associated with the project.
• To attend, contribute, and where necessary lead relevant meetings.
• To ensure that responsibilities identified within internal processes such as Health and Safety guidelines etc are robustly met by all research team members.
• To take responsibility for planning and developing research methods and techniques within the framework of the research programme.
• To contribute to relevant teaching activities within the department.
• To undertake any other duties relevant to the programme of research.
• A PhD in a in a basic or health science
• Research experience in the relevant subject area of the research project
• Experience of working in a research team environment
• Experience of writing research reports, publications or other relevant outputs
• Previous teaching experience
Skills & Knowledge Essential:
• Applied research experience in own area or specialism
• Excellent interpersonal, networking and communication skills
• Proven problem solving skills
• Excellent ICT skills
• A good understanding of the research environment in a university
• Experience of writing to publication standard or independently producing other research outputs
Personal Qualities Essential:
• A clear commitment to contributing to the research environment and producing high quality research outputs
• Proactive independent attitude
• Flexible and adaptive with a positive approach to managing substantial projects and associated resources
• A committed team-player