Postdoctoral Research Scientist - Stem cells and disease modeling
- Columbia University
- Location: New York, NY
- Job Number: 7126621
- Posting Date: Feb 26, 2023
- Salary / Pay Rate: $65,000
- Application Deadline: Open Until Filled
The lab applies human induced pluripotent stem cells to model neurodegenerative diseases, with a special emphasis on tauopathies including frontotemporal dementia and Alzheimer’s disease. Current studies within the lab focus on elucidating the mechanisms of disturbed function in patient neurons. We apply cell stress, seahorse and metabolomics assays, bulk and single cell sequencing, and co-culture assays using iPSC-derived neurons and glial cells including astrocytes and microglia to characterize cell-intrinsic disease phenotypes as well as non-cell-autonomous mechanisms of disease development. Furthermore, we transplant patient and control iPSC-derived neural cells into the brains of immunocompromised mice followed by histology and snRNA-seq of microdissected grafts. These studies are complemented by snRNA-seq studies on postmortem brain tissue from patients with tauopathies carrying the same disease-associated mutations as our iPSCs.
Experimental techniques for this project include:
· Culture and differentiation of human induced pluripotent stem cells into neurons and glial cells
· In vitro assays on differentiated cells
· Transplantation of iPSC-derived neurons into the brains of immunocompromised mice
· Histology of grafts followed by confocal imaging
· Microdissection of grafted human neurons followed by snRNA-seq
The position is available full-time.
Experience with human stem cell culture and differentiation is preferred.
Career development including attendance at conferences is strongly supported.
Start date: 4/1/2023
Please send your CV and cover letter to Dr. Gunnar Hargus: [email protected]
At Columbia University, we stand together because diverse experiences, perspective, and values enrich every dimension of our work. All qualified applicants will receive consideration for employment independent of race, ethnicity, religion, creed, sexual orientation, gender identity or expression, national origin, age, disability, or any other factor which cannot be used as a basis for employment decision.
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