Decipher the network of proteins, genes and enhancers that drive T-cell leukemia and regeneration in a university #3 in research and #1 for livability!


Job summary:
The University of Michigan in Ann Arbor ranks #3 in the United States in research and development expenditures at ~$1.5 billion annually. Ann Arbor is a mix of city and rural spaces and is ranked on several lists as the #1 or #2 place to live especially for affordable living and for families. Public school, arts and culture, restaurants, and outdoor green spaces are highly ranked. Spend only a 5-minute walk or a up to a 10-minute drive to work instead of 1 hour.
https://www.mlive.com/life/2023/02/michigan-college-town-ranked-the-no-1-best-place-to-live-in-america-in-2023.html
https://fortune.com/well/ranking/best-places-families/2022/ann-arbor/

Acute lymphoblastic leukemia (ALL) kills more children than any other cancer. Our goal is use cutting-edge transcriptional genomic and proteomic technologies and sophisticated preclinical animal and human models to identify and elucidate the network of transcriptional regulators, genes and regulatory elements of ALL and regenerating T-cells in order to develop novel targeted anti-leukemic therapies that are more effective and less toxic than current medicines. We will also apply this knowledge towards developing medicines that promote recovery of T-cell immunity after cytoreductive therapy.
Lab website: http://chiangmarky8.wix.com/chiang-lab

Specific methodologies:
-Leukemogenesis and T-cell development assays (flow cytometry sorting and analysis; histology; bone marrow transplantation; intrathymic injections; retroviral transduction and transplantation; in vitro stromal cell culture; transplantation in NSG mice)
-Transcriptional genomics (ChIP-Seq/ATAC/4C-Seq library preparation; analyses of RNA/ChIP/ATAC-seq datasets using computational pipelines; bioinformatics)
-Transcription factor/enhancer functional screens and assays (CRISPRi; ChIP; MPRAs)
-Protein-protein interaction biochemistry (mass spectrometry; BIOID; NMR; co-IP; reverse-ChIP)

Model systems:
- Genetically engineered mouse models
- Human leukemia patient-derived xenograft models
- Human and mouse leukemia cell lines

Specific responsibilities:
-Design, perform, analyze, and interpret experiments with training and supervision from PI and other lab members in line with skill set and with goal of independence
-Work as a team with other lab members (PI, bioinformaticist, postdocs, PhD students, and the lab manager/work-study students)
-Collaborate with members of other labs
-Write manuscripts for publication
-Present posters and talks at research conferences

Required Qualifications:
#PhD in a biomedical field

Please send your CV to:
[email protected]

Keywords:
#postdoc
#postdoctoral fellow
#research fellow
#Notch
#T-ALL
#MYC
#MYB
#CRISPRi screens
#Mass spectrometry
#Massively Parallel Reporter Assay screens
#trasncriptional networks
#enhancer
#super-enhancer
#pediatric cancer
#immunology
#cancer biology
#transcription factors
#mouse models
#PDX
#T-cells
#T-cell leukemia
#T-lymphocytes
#thymus
#hematology
#hematological malignancies
#hematopoiesis
#lymphopoiesis
#regenerative medicine
#stem cells
#T-cell regeneration