Postdoctoral Appointee – Large scale RNA production for Structural Biology of RNA
- Argonne National Laboratory
- Location: Lemont, USA
- Job Number: 7322709 (Ref #: 421483)
- Posting Date: 1 month ago
Job Description
We invite applications for a postdoctoral position in working at Advanced Protein Characterization Facility (APCF) in the X-ray Science Division at Argonne National Laboratory. The selected candidate will participate in RNA project for structural characterization of RNA by SAXS, x-ray crystallography, and Cryo-EM.
The main role for the project is to produce RNA samples in large scale by using in vitro transcription with T7 RNA polymerase, subsequent purification necessary for SAXS, MX and Cryo-EM. The work requires experience in molecular biology for basic cloning and protein expression (utilizing E. coli), nucleic acid and protein purification. Preparation of proteins needed during RNA production, such nucleases, phosphatase, RNase inhibitors as well as RNA chaperones used for crystallization are also part of duty. Involvement in the research pertinent to specific RNA targets will be expected as well, along with presentation of findings to the community through publications and presentations.
Position Requirements
Ph.D. completed in the past 5 years or soon to be completed in Molecular Biology, Biochemistry, Structural Biology, Biotechnology, Protein Engineering, Microbiology, or a related field
Strong molecular biology skills (cloning, vector design, transformation), protein and nucleic acid prep-scale purification and analysis, and quantitative data analysis
Excellent communication and teamwork capabilities, with a track record (or potential) in writing publications and presenting scientific results
Ability to model Argonne’s core values of impact, safety, respect, integrity, and teamwork
Interpersonal skills, oral and written communication skills, and ability to interact with people at all levels both within and outside the laboratory
Preferred Knowledge, Skills, and Experience
Knowledge of in RNA biology
Experience with RNA CryoEM/crystallography/SAXS
Prior experience with high-throughput or computational protein design/screening techniques
Job Family
PostdoctoralJob Profile
Postdoctoral AppointeeWorker Type
Long-Term (Fixed Term)Time Type
Full timeThe expected hiring range for this position is $72,879.00-$121,465.00.Please note that the pay range information is a general guideline only. The pay offered to a selected candidate will be determined based on factors such as, but not limited to, the scope and responsibilities of the position, the qualifications of the selected candidate, business considerations, internal equity, and external market pay for comparable jobs. Additionally, comprehensive benefits are part of the total rewards package.
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